Objective To investigate the cognition,attitude and practice of pediatric ICU nurses on daily sedation interruption（DSI）,and to provide references for clinical training. Methods Convenience sampling was used to conduct a questionnaire survey among 298 pediatric ICU nurses in 7 tertiary A children hospitals in China in July 2021. The general information questionnaire,questionnaires of knowledge,attitude,and behavior on DSI were self-designed. Results In terms of the cognition of 298 pediatric nurses on DSI,their knowledge score was 4（2,4） out of 10,and a total of 157 nurses（52.68%） said they did not know well or had never heard of DSI. In terms of attitude,their recognition score was 32（27,34） out of 40,and 217 nurses（72.82%） were willing to implement DSI,and 258 nurses（86.58%） wanted systematic training. In practice of nurses,221（74.16%） have not received relevant training,and 159 nurses（53.35%） never or rarely performed DSI. Conclusion Pediatric ICU nurses have a poor grasp of knowledge on DSI,while their recognition toward DSI is high. Their practice needs to be strengthened. Nursing managers should pay full attention to the training needs of pediatric ICU nurses and carry out systematic training on DSI basic knowledge and practical skills,so as to improve DSI practice level of nurses.